KMID : 0525720180230020069
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Journal of Chitin and Chitosan 2018 Volume.23 No. 2 p.69 ~ p.75
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Preparation of Water-Soluble Chitosan and Aloe vera Mucilage and Their Use for Assembly of Polyelectrolyte Complexes
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Rangel-Rodriguez Adriana M.
Adriana Sanudo-Barajas J. Balagurusamy Nagamani Wicker Louise Velez-de-la-Rocha Rosabel Contreras-Esquivel Juan Carlos
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Abstract
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In this work, the preparation and characterization of water-soluble polysaccharides from chitosan and Aloe vera was studied. The water-soluble polysaccharides were used to study polyelectrolyte complexes. The reaction time effect for chitosan hydrolysis by endo-chitosanase was studied at 40¨¬C and pH 5.00 to produce water soluble chitosan (WSCh). The physico-chemical characteristics of chitosan hydrolysates, water-soluble A. vera polysaccharides and polyelectrolyte complexes were determined. After 3 h of chitosan processing, a viscosity reduction of 90%, while only 2.3% of reducing sugars were released. A WSCh was recover by ultrafiltration (1 kDa) from chitosan hydrolysate after 12 h and was spray-dried with a yield of 9.7%. Cold-water extraction of A. vera mucilage from pulp gives a crude polysaccharide yield of 0.81 g/kg (wet basis) based on whole leaf weight. The water isolated mucilage is composed of a mixture of protein and mannan rich-polysaccharide. The results show WSCh¡¯s association capacity with A. vera mucilage by electrostatic interaction.
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KEYWORD
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Acemannan, Pectic substances, Chitosanase, Remazol brilliant blue, Spray-dyer
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